This ELISA kit is used for quantitative determination of Smad1 in rat tissue homogenates or other relevant biological fluids
This manual provides detailed guidance for performing the SMAD1 ELISA assay. It lists all required components, describes preparation of standards and reagents, and outlines the step‑by‑step protocol for sample incubation, washing, and detection.
Additional sections cover storage conditions for reagents, plate handling, washing methods, data analysis, and safety warnings. The document is intended for laboratory personnel conducting quantitative SMAD1 measurements. This manual is written in Japanese.
| Detection range | 0.312 ng/mL – 20 ng/mL |
|---|---|
| Minimum detection limit | 0.078 ng/mL |
| Plate type | 96‑well microplate |
| Incubation temperature | 37°C |
| Reagent storage temperature | -20°C (sealed with desiccant) |
The kit can detect SMAD1 down to 0.078 ng/mL.
Incubate the plate at 37 °C for the specified time.
Store reagents at –20 °C in sealed containers with desiccant; avoid moisture.
Add at least 0.4 mL wash buffer to each well, soak 1–2 minutes, then discard; repeat as described.