ReverTra Ace® qPCR RT Kit is a high-efficiency and user-friendly reverse transcription reaction kit for making Realtime PCR template cDNA. This product uses high-performance reverse transcriptase ReverTra Ace® and optimized reaction components for short-chain cDNA synthesis as Realtime PCR targets, which can efficiently synthesize cDNA templates suitable for Realtime PCR.
The ReverTra Ace qPCR RT Kit is a high‑efficiency, easy‑to‑use kit for synthesizing cDNA templates for real‑time PCR. It contains a 5X RT Buffer, a high‑performance reverse transcriptase enzyme mix, a primer mix (Random Primer + Oligo(dT) Primer), and nuclease‑free water, all stored at –20 °C.
The manual details the required additional items (thermal cycler, incubator, extra nuclease‑free water), step‑by‑step reverse transcription (37 °C for 15 min, 98 °C for 5 min enzyme inactivation), and guidelines for adding the cDNA to PCR reactions (max 20 % of the PCR volume). It also includes a FAQ section and references to related products. This manual is written in Chinese.
| Storage Temperature | -20 °C for all reagents |
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Yes, the cDNA can be added directly, but it should not exceed 20 % of the PCR reaction volume.
A thermal cycler, an incubator, and additional nuclease‑free water are required.