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- Languages
- ZH
- Last added
- 2011-11-12
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TOYOBO ReverTra Ace qPCR RT Kit FSQ-101 User Manual
The ReverTra Ace qPCR RT Kit is a high‑efficiency, easy‑to‑use kit for synthesizing cDNA templates for real‑time PCR. It contains a 5X RT Buffer, a high‑performance reverse transcriptase enzyme mix, a primer mix (Random Primer + Oligo(dT) Primer), and nuclease‑free water, all stored at –20 °C.
The manual details the required additional items (thermal cycler, incubator, extra nuclease‑free water), step‑by‑step reverse transcription (37 °C for 15 min, 98 °C for 5 min enzyme inactivation), and guidelines for adding the cDNA to PCR reactions (max 20 % of the PCR volume). It also includes a FAQ section and references to related products. This manual is written in Chinese.
Covers: Preface · Product Contents · Other Required Items · Usage Method · Related Operation Steps · Frequently Asked Questions
Latest TOYONBO manuals
TOYOBO ReverTra Ace qPCR RT Kit FSQ-101 User Manual
User Manual 12 pages · 302 KB · ZHThe TOYOBO ReverTra Ace qPCR RT Kit (FSQ-101) user manual provides reagent contents, preparation steps, and troubleshooting for efficient cDNA synthesis.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in TOYONBO manuals
Can the cDNA produced by the kit be directly added to a real‑time PCR reaction?
Yes, the cDNA can be added directly, but it should not exceed 20 % of the PCR reaction volume.
What additional equipment or reagents are required besides the kit contents?
A thermal cycler, an incubator, and additional nuclease‑free water are required.