Agilent Manuals

Eclipse XDB-C8 and -C18 are microparticulate packings that use the technologies of eXtra-Dense Bonding (XDB) of organo-silane ligands and double endcapping to protect the ultra-pure (Type B) silica support from dissolution in mobile phases of intermediate pH. Eclipse XDB-C8 and -C18 are especially useful for the separation of acidic, basic, and other highly polar compounds by reversed-phase liquid chromatography. Eclipse XDB-C8 and -C18 are made by first chemically bonding a dense monolayer of dimethyloctyl (or octadecyl) silane stationary phase to a specially prepared, ultra-high purity ( 99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special ZORBAX silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded phase packing is then double endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse XDB-C8 and -C18 can be used in the pH range of 2–9, but is best suited for long-term operation at pH 3–8. The uniform, spherical Eclipse XDB-C8 and -C18 particles are based on ZORBAX Rx-SIL silica support that has a surface area of 180 m2/g and a controlled pore size of 80Å. Columns are packed to a stable uniform bed density using a proprietary, high-pressure, slurry-packing technique to give maximum column efficiency and longevity.

The Agilent ZORBAX SB-C18 RRHT Threaded Column is designed for high-pressure operation (up to 600 bar) and is packed with a high-performance microparticulate C18 packing for high-speed reversed phase HPLC. The StableBond packing is made by chemically bonding a sterically-protected C18 stationary phase to a specially prepared, high purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected, diisobutyl n-octadecylsilane stationary phase is chemically stable and gives longer column life. As a result, ZORBAX SB-C18 is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well suited for use with aggressive mobile phases (for example, pH <2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 90 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-C18 is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-C18 particles have a controlled pore size of 80Å.

This document is the datasheet of Agilent Eclipse XDB-C18 RRHT Threaded Column

Agilent ZORBAX Extend-C18 RRHT threaded column for high pH and high pressure liquid chromatography

Agilent ZORBAX Eclipse XDB-C8 RRHT Threaded Column is a high-speed reversed-phase HPLC column designed for higher pressure operation (up to 600 bar) and packed with a high-performance microparticulate C8 packing. The column utilizes the eXtra-Dense Bonding (XDB) technology to bond organo-silane ligands to an ultra-pure silica support, and employs double endcapping to protect the support from dissolution in mobile phases of intermediate and higher pH. It is especially useful for the separation of acidic, basic, and other highly polar compounds by reversed-phase liquid chromatography.

Agilent ZORBAX SB-C8 RRHT threaded columns are specially designed for higher pressure operation and are packed with a high-performance microparticulate C8 packing for high-speed reversed-phase HPLC. The StableBond packing is made by chemically-bonding a sterically-protected C8 stationary phase to a specially-prepared, high-purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely-covered, sterically-protected, diisopropyl n-octylsilane stationary phase is chemically stable and gives longer column life. It is particularly well-suited for use with aggressive mobile phases (for example, pH <2, high ionic strength, ion- pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-C8 is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-C8 particles have a controlled pore size of 80Å. The columns are 250 mm long, 4.6 mm inner diameter, and the particle size is 5 μm.

Agilent ZORBAX SB-CN RRHT threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high-performance microparticulate CN packing for high-speed reversed- phase HPLC. The StableBond packing is made by chemically bonding a sterically-protected CN stationary phase to a specially-prepared, high-purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely-covered, sterically-protected, diisopropyl cyanopropylsilane stationary phase is chemically stable and gives longer column life. As a result, ZORBAX SB-CN is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well-suited for use with aggressive mobile phases (for example, pH <2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long- term stability and reproducibility. ZORBAX SB-CN is especially suited in applications that use high- sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-CN particles have a controlled pore size of 80Å.

Eclipse Plus C18 RRHT threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high performance microparticulate C18 packing for high-speed reverse phase HPLC. Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 packing is made by first chemically bonding a dense monolayer of dimethyl-n-octadecylsilane stationary phase to a specially prepared, an improved ultra-high purity (≥99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special ZORBAX silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar com- pounds. The bonded-phase packing is then doubly end- capped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C18 columns can be used for acidic and neutral sam- ples, but are especially suited for separations of basic com- pounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2–9, accommodating most popular mobile phases. The uniform, spherical Eclipse Plus C18 particles are based on an improved ZORBAX Rx-SIL support that has a nomi- nal surface area of 160 m2/g and a controlled pore size of 95Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure slurry-loading t

Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 is especially useful for the separation of acidic, basic, and other highly polar compounds by reverse-phase liquid chromatography. Eclipse Plus C18 packing is made by first chemically bonding a dense monolayer of dimethyl-n-octadecylsilane stationary phase to a specially prepared, an improved ultra-high purity (>99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded-phase packing is then doubly endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C18 columns can be used for acidic and neutral samples, but are especially suited for separating basic compounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2–9, accommodating most popular mobile phases. The uniform, spherical, Eclipse Plus C18 particles are based on an improved ZORBAX Rx-SIL support that has a nominal surface area of 160 m²/g and a controlled pore size of 95Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure slurry-loading technique to give maximum column efficiency.

Eclipse Plus C8 RRHT threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high-performance microparticulate C8 packing for high-speed reverse-phase HPLC. Eclipse Plus C8 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C8 packing is made by first chemically bonding a dense monolayer of dimethyl-n-octylsilane stationary phase to a specially pre- pared and improved ultra-high purity (≥99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special ZORBAX silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar com- pounds. The bonded-phase packing is then doubly end- capped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C8 columns can be used for acidic and neutral sam- ples, but are especially suited for separations of basic com- pounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2 to 8, accommodating most popu- lar mobile phases. The uniform, spherical Eclipse Plus C8 particles are based on an improved ZORBAX Rx-SIL support that has a nomi- nal surface area of 160 m2/g and a controlled pore size of 95Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure slurry-loading tech

ZORBAX SB-AQ RRHT threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high-performance microparticulate packing for high-speed reversed-phase HPLC. The StableBond AQ packing is made by chemically bonding a reversed-phase proprietary alkyl group to a specially prepared, high-purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected stationary phase is chemically stable and gives longer column life. As a result, ZORBAX SB-AQ is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well-suited for use with aggressive mobile phases (for example, pH < 2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-AQ is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-AQ particles have a controlled pore size of 80Å. Columns are loaded to a uniform Agilent ZORBAX SB-AQ RRHT Threaded Column Data Sheet be

Eclipse Plus C8 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types.

The ZORBAX Rx-Sil RRHT threaded column is specially designed for higher pressure operation (up to 600 bar) and is packed with a high-performance microparticulate silica packing for high-speed liquid-solid adsorption chromatography. ZORBAX Rx-Sil particles are produced by the agglomeration of colloidal silica to form spherical particles of uniform diameter and pore size. However, ZORBAX Rx-Sil is specially treated to create a highly homogeneous sur- face throughout the silica support. This homogeneity is achieved by utilizing ultra-high-purity silica (less than 100 ppm total of impurities such as sodium, aluminum, iron, etc.) and by applying patented technology for fully hydroxylating the silica surface. As a result, ZORBAX Rx-Sil can be used for basic, neutral, or acidic samples. It is particularly well suited for use with basic samples, since these solutes can be chromatographed without peak tailing or irreversible adsorption. The uniform, spherical, ZORBAX Rx-Sil particles have a controlled pore size of 80Å. Columns are loaded to a uni- form bed density using a proprietary, high-pressure, slurry-loading technique to give optimum column efficiency.