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- 2026-11-06
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Kaitai Bio AT3013-01/02 FFPE Tissue DNA Extraction Kit User Manual
This manual provides step-by-step instructions for extracting genomic DNA from FFPE tissue samples using the AT3013-01/02 kit. It includes protocols for both non-toxic and xylene-based wax removal, sample preparation, enzymatic digestion, column-based purification, and DNA elution. Safety precautions and troubleshooting tips are included for common issues like low yield or RNA contamination.
The kit contains buffers, enzymes, and spin columns for DNA extraction, with optional reagents like RNaseA for RNA removal. It is designed for downstream applications including PCR, NGS, SNP, STR analysis, and pharmacogenomics. This manual is written in Chinese.
Covers: Kit Components · Sample Preparation · Wax Removal (Non-toxic or Xylene) · DNA Extraction Steps · RNA Removal · DNA Elution
Latest 开泰生物 manuals
Kaitai Bio AT3013-01/02 FFPE Tissue DNA Extraction Kit User Manual
User Manual 5 pages · 437 KB · ZHUser manual for Kaitai Bio's FFPE tissue DNA extraction kit, detailing steps for safe, toxin-free DNA isolation from paraffin-embedded samples.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in 开泰生物 manuals
How do I remove RNA contamination?
Add 2–5 μL RNaseA (100 mg/mL) to the digest solution after 90°C de-crosslinking, mix, and incubate at room temperature for 15 minutes.
From: Kaitai Bio AT3013-01/02 FFPE Tissue DNA Extraction Kit User Manual
Can I shorten incubation times to speed up the process?
Yes, incubation times can be reduced: 56°C lysis to 30 min, 90°C denaturation to 30 min. DNA yield may decrease slightly but is still suitable for most applications.
From: Kaitai Bio AT3013-01/02 FFPE Tissue DNA Extraction Kit User Manual