Instructions for handling, culturing, freezing, and troubleshooting the PT67 reverse‑transcriptase virus packaging cell line (U30034).
This manual provides detailed information on the PT67 reverse‑transcriptase virus packaging cell (catalog number U30034) supplied by Yobibio. It covers cell origin, species, growth characteristics, recommended culture media, temperature, CO₂ conditions, and storage requirements.
Step‑by‑step passaging procedures, freezing guidelines, supply restrictions, and common troubleshooting tips are included to help researchers maintain healthy cultures and resolve typical issues such as bottle leakage or cell floating. This manual is written in Chinese.
| Cell Source | ATCC / Chinese Academy of Sciences / Union Hospital |
|---|---|
| Growth Characteristic | Adherent (贴壁) |
| Culture Medium | 90% DMEM(H) + 10% FBS |
| Recommended Serum | UBI U11‑059A (特级胎牛血清) |
| CO₂ Level | 5 % (95 % air, 5 % CO₂) |
| Long‑Term Storage | Liquid nitrogen |
The cell culture may be contaminated; Yobibio will arrange technical assistance to resolve the issue.
If you must keep the original medium, add 10 % serum and use it for no more than 72 hours.
If most cells re‑attach to the bottle bottom after 1–2 hours, the remaining floating cells can be removed and the culture continued.