This manual provides instructions for the culture, passaging, freezing, and revival of the RAMOS-LUC human B lymphoma cell line, including specific media…
This document details the basic information and culture conditions for the RAMOS-LUC human B lymphoma cell line. It specifies that the cells are of human origin, exhibit a lymphoblastoid morphology, and grow in suspension. The recommended culture medium consists of 90% RPMI-1640, 10% FBS, and 1% PS, maintained at 37°C in an atmosphere of 95% air and 5% CO2. This manual is written in Chinese.
| Cell Line Name | RAMOS |
|---|---|
| Tissue Origin | Lymph |
| Cell Morphology | Lymphoblastoid |
| Growth Characteristic | Suspension |
| Culture Medium | 90%RPMI-1640+10%FBS+1%PS |
| CO2 Requirement | 5% |
| Passage Ratio | 1:2 to 1:3 |
| Freezing Condition | Serum-free freezing medium, liquid nitrogen storage |
| Puromycin Selection Concentration | 0.5 ug/ml |
The recommended passage ratio is 1:2 to 1:3.
Cells should be mixed with serum-free freezing medium at a density of 5×106~1×107/mL, placed in a -80°C freezer for 24 hours, and then transferred to liquid nitrogen.
No, the transport medium (inoculation medium) cannot be used for cell culture; fresh complete medium must be prepared according to the manual.