The TOSOH TSK-GEL Amide-80 Series instruction manual provides safety guidelines, installation steps, operation tips, maintenance procedures, and…
This manual explains the safe handling of TSK‑GEL Amide‑80 packed columns, including fire‑hazard warnings, ventilation requirements, and personal protective equipment. It also details the required documentation that should accompany each column shipment.
The guide covers column installation, recommended flow rates, solvent preparation, temperature limits, routine maintenance, guard‑column replacement, and troubleshooting of common issues such as clogging or loss of resolution. Technical specifications and warranty information are provided in the final sections.
| pH range | 2.0‑7.5 |
|---|---|
| Recommended linear velocity | 6 cm/min (0.05 mL/min for 1.0 mm I.D., 0.20 mL/min for 2.0 mm I.D., 0.43 mL/min for 3.0 mm I.D., 1.00 mL/min for 4.6 mm I.D.) |
| Column dimension example | 4.6 mm I.D. × 25 cm (TSK‑gel Amide‑80 5 µm) |
Keep the columns away from fire, use them only in well‑ventilated areas, and wear protective eye gear and gloves to avoid skin contact with organic solvents.
Connect the union nut to a 1/16" O.D. capillary tubing, confirm the flow direction on the column label, purge air, and start the solvent at half the intended flow rate.
Set a linear velocity of 6 cm/min, which corresponds to 0.05 mL/min for a 1.0 mm I.D. column, 0.20 mL/min for 2.0 mm I.D., 0.43 mL/min for 3.0 mm I.D., and 1.00 mL/min for 4.6 mm I.D.
Purge the system with the packed solvent, cap both ends of the column, and store it at a relatively constant temperature, avoiding freezing.