The Tian En Ze 16-99183 LAMP Kit User Manual provides step‑by‑step instructions for sample preparation, reaction setup, and result interpretation of the 木蝴蝶…
The 木蝴蝶 LAMP Kit (model 16-99183) is a research‑only diagnostic kit that uses isothermal LAMP amplification to detect Semen oroxyli DNA. It includes 2× visual UNG‑LAMP MagicMix, primer mix, a positive control, and a user manual, and is designed for field or low‑resource laboratory use.
The manual describes how to prepare DNA from samples, set up a 20 µL LAMP reaction with the provided reagents, incubate at 61 °C for 60 min (with a 5 min pre‑incubation at 25 °C), and interpret results by color change. It also covers optional gel electrophoresis verification, storage conditions, and precautions to avoid contamination. This manual is written in Chinese.
| Storage temperature | -20 °C |
|---|---|
| Shelf life | 1 year |
| Reaction volume | 20 µL |
| Number of reactions per kit | 50 |
| Minimum detection sensitivity | ≈100 copies per reaction |
| Positive control concentration | 1 × 10⁸ copies/µL |
One kit can perform 50 reactions of 20 µL each.
The kit should be stored at -20 °C.
The kit can detect as few as about 100 DNA copies per reaction.