This application note details the automated production and growth of single spheroids in Corning 96-well microplates using the Tecan Fluent Automation…
The document describes an experiment utilizing the Fluent Automation Workstation to automate cell seeding and medium exchange for 3D cell culture. The system, equipped with an eight-channel air-displacement Flexible Channel Arm, a long Z Robotic Gripper Arm, and a Multiple Channel Arm (MCA), was used to seed human fibroblasts and murine embryonal fibroblasts in ultra-low attachment Corning microplates.
Results demonstrated that the automated process successfully formed single spheroids with morphology and dimensions comparable to manual methods. Crucially, automated medium exchange prevented the loss of spheroids observed during manual exchanges, ensuring high reproducibility and maintaining a sterile environment without contamination issues over a 14-day culture period.
The MCA with an extended volume adapter can pipette up to 500 μl.
The system allows medium exchange for all 96 wells in parallel.
The system uses a low aspiration speed of 1 µl/s.