The StageTips application manual provides step‑by‑step protocols for peptide and protein purification using C18, C8, and strong cation exchange micro‑columns.
StageTips are disposable polypropylene tips packed with PTFE‑supported chromatographic particles. They are compatible with standard pipettes and most commercial automation systems, and are offered with C18, C8, or strong cation exchange (SCX) resin.
The manual describes activation, equilibration, sample loading, washing, and elution procedures for each resin type, including specific solvent volumes and concentrations. It also gives practical tips for avoiding over‑pressure, handling larger sample loads, and preparing eluates for downstream mass‑spectrometry applications. This manual is written in Chinese.
| Sample capacity (smallest C18 StageTips) | ≈ 3 µg protein or peptide (~50 pmol BSA) |
|---|---|
| Tip material | Disposable polypropylene tip with PTFE‑supported resin |
Use 10 µL of 50 % methanol / 5 % formic acid to activate the column.
Approximately 3 µg of protein or peptide, which corresponds to about 50 pmol of BSA.
Wash with 10–30 µL of 50 % methanol / 5 % formic acid, then equilibrate with the appropriate A or B buffer.