This manual provides instructions for using the Sanxiang 2019-nCoV nucleic acid detection kit for qualitative diagnosis of SARS-CoV-2 via fluorescent PCR.
The Sanxiang 2019-nCoV Nucleic Acid Detection Kit (Fluorescent PCR) is designed for the in vitro qualitative detection of SARS-CoV-2 ORF1ab and N genes in pharyngeal swab and alveolar lavage fluid samples. The kit utilizes real-time fluorescent quantitative RT-PCR technology with dual-target gene design and an internal control to monitor sample collection and extraction processes.
The manual details the kit's composition, storage conditions, and specific requirements for sample types and handling. It outlines the step-by-step inspection method, including reagent preparation, sample processing, PCR amplification parameters, and result analysis. Quality control criteria for positive and negative controls are specified to ensure valid experimental results. This manual is written in Chinese.
| Packaging Specification | 24 tests/box |
|---|---|
| Minimum Detection Limit | 200 copies/mL |
| Applicable Instrument | SLAN-96P |
| Sample Types | Pharyngeal swab, Alveolar lavage fluid |
| Intrabatch Precision CV | ≤5% |
The minimum detection limit of this kit is 200 copies/mL.
For the negative control, there should be no Ct value in the FAM, ROX, or internal standard (HEX) channels, or the Ct value must be greater than 40.
A sample is reported as positive if a typical S-shaped amplification curve is detected in the FAM or ROX channel with a Ct value less than or equal to 40.