Rainbow 180 Protein Marker provides 10 pre‑stained bands from 11 kDa to 180 kDa for SDS‑PAGE and Western blot analysis.
The Rainbow 180 Protein Marker contains ten colored, pre‑stained protein standards covering a molecular weight range of 11 kDa‑180 kDa. Each band contains about 0.2‑0.4 mg/ml of protein and is designed for clear visualization on SDS‑PAGE gels and transferred membranes.
The marker is supplied as a ready‑to‑use liquid; 5 µl per lane is sufficient for clear band detection. Store the solution at –20 °C (stable for at least two years) and keep it at 4 °C if ice forms. Use a 15 % separating gel for optimal resolution of bands below 25 kDa. This manual is written in Chinese.
| Molecular weight range | 11 kDa–180 kDa |
|---|---|
| Number of bands | 10 colored bands |
| Protein amount per band | 0.2–0.4 mg/ml |
| Recommended sample volume | 5 µl |
| Shelf life | at least 2 years |
| Recommended gel concentration | 15 % |
| Available formats | 50 µL (250 µL×2), 100 µL (250 µL×2), 500 µL (250 µL×10) |
It covers proteins from 11 kDa up to 180 kDa.
Only 5 µl is needed for clear band visualization.
Store at –20 °C; if ice forms, split into aliquots and keep at 4 °C for at least six months.
A 15 % separating gel is recommended.