Detailed user manual for the QuantiCyto EHC170 ELISA kit that detects human IL‑17/IL‑17A in research samples.
The QuantiCyto EHC170 ELISA kit uses a double‑antibody sandwich format to quantitatively measure human IL‑17/IL‑17A in serum, plasma or cell‑culture supernatants. The manual provides a complete list of kit components, storage conditions, and step‑by‑step instructions for sample preparation, reagent handling, plate washing, incubation, and result interpretation.
The guide also includes troubleshooting tips, sensitivity and specificity data, and recommended equipment such as a 450 nm plate reader and precision pipettes. All procedures are intended for research use only and must be performed under appropriate biosafety conditions. This manual is written in Chinese.
| Sensitivity | 7.8 pg/ml |
|---|---|
| Specificity | No cross‑reactivity with IL‑1,2,3,4,5,6,7,8,9,10,11,12,13,17B,17C,17E,17F, IFN‑γ, TNF, TGF, MCP‑1, ANG, AR, CNTF, EGF, Epo, VEGF, OSM |
| Reproducibility | Intra‑ and inter‑plate CV < 10 % |
| Storage Temperature (Reagents) | 2 °C – 8 °C |
| Sample Storage | -20 °C – -70 °C (up to 3‑6 months) |