A 2.5x concentrated ready-reaction premix kit for Sanger sequencing of PCR products and plasmids, optimized for long, high-quality reads.
The BrilliantDye v1.1 Terminator Cycle Sequencing Kit is a complete kit based on the Sanger Chain Termination method. It is delivered as a 2.5x concentrated ready-reaction premix, fully optimized for a highly flexible chemistry, designed for all kinds of Sequencing applications, including de novo sequencing and resequencing. The kit generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads.
| Concentration | 2.5x concentrated |
|---|---|
| Method | Sanger Chain Termination |
| Buffer Concentration | 5X |
| Recommended Primer Concentration | 3.2 – 5 µM |
| Recommended Primer Amount | 3 - 5 pMol |
| Recommended Premix Volume | 8 µL |
The kit is designed for all kinds of Sequencing applications, including de novo sequencing and resequencing.
The recommended template quantity is 1–3 ng.
The recommended template quantity is 150–300 ng.
Recommended platforms include Applied Biosystems 310, 3100, 3130, 3500, 3730, SeqStudio, and Promega Spectrum Compact CE.