The Nikon TS100 DS-L3 user manual provides safety notes, setup steps, operation instructions, and troubleshooting for the inverted microscope.
This manual explains the purpose and principle of the Nikon TS100 DS-L3 inverted microscope, describing how phase‑contrast and fluorescence imaging are achieved. It lists the required samples, illumination options, and the components that make up the system, including the microscope body, eyepiece, objective, condenser, phase plates, and fluorescence module.
Detailed operating procedures cover powering the microscope and CCD, selecting the appropriate phase ring or fluorescence filter, adjusting illumination intensity, and capturing images. The guide also includes a troubleshooting section that helps resolve common issues such as no light output, dark fields, or stray shadows during observation. This manual is written in Chinese.
| Eyepiece field number | 22 mm |
|---|---|
| Objective type | Plan Fluor with phase‑contrast capability |
| Fluorescence filter set | UV‑2E/C (DAPI), B‑2A (green), G‑2A (red) |
| Fluorescence power‑supply lifetime | ≈200 hours |
| Minimum fluorescence illumination time per session | 30 minutes |
Check whether the condenser aperture is closed, which can block the light path.
Ensure the beam splitter is positioned for binocular viewing and that the objective is correctly rotated into position.
Wait about 3–5 minutes after turning on the lamp for the light source to stabilize before starting observation.
Allow at least 5 minutes to pass after switching off the fluorescence lamp to extend its lifespan.