The MultiSciences Biotech AP105 Apoptosis Kit manual provides background, components, step‑by‑step usage instructions, result examples and safety…
This manual introduces the Annexin V‑APC/7‑AAD Apoptosis Kit, explaining how fluorescently labeled Annexin V binds phosphatidylserine on apoptotic cells, enabling rapid detection within 15 minutes. It also lists the kit's components and storage requirements.
Detailed protocols cover sample preparation, staining procedures, flow cytometer setup, result interpretation, and important safety notes. The document ends with example data and references to related products. This manual is written in Chinese.
| Available formats | 30T, 60T, 100T |
|---|---|
| Storage temperature | 2‑8 °C, protected from light, do not freeze |
| Storage solution | Phosphate buffer with 0.09 % NaN₃, pH 7.2 |
Fluorescently labeled Annexin V specifically binds phosphatidylserine on the surface of apoptotic cells, indicating cell death.
Store the kit at 2‑8 °C, protected from light, and do not freeze it.
Add 5 µl Annexin V‑APC or 10 µl 7‑AAD to each tube, incubate in the dark at room temperature for 5 minutes, then analyze by flow cytometry.