User manual for Merckinade SDA-501, a water-in-oil-in-water emulsion adjuvant for veterinary vaccines, covering usage, sterilization, and emulsification…
The Merckinade SDA-501 is a self-emulsifying antigen adjuvant system designed for veterinary vaccines. It is a water-in-oil-in-water emulsion containing a very low amount of oil phase, offering high stability and safety. The product is suitable for various vaccine types, including bacterial, mycoplasma, inactivated viral, vector, recombinant gene, and live vaccines, helping to improve immune responses while reducing side effects and toxicity. This manual is written in Chinese.
| Oil content in final emulsion | 6.5% |
|---|---|
| Water phase antigen proportion | 90% |
| Recommended antigen to adjuvant ratio | 1:4 to 1:9 |
| Storage temperature | 5~8 ℃ |
| Average particle size | 150 nm |
| Viscosity (capillary method) | < 6 seconds for 0.4 mL |
| Centrifugation stability (3000 rpm/min, 15 min) | Water phase separation < 0.5 mL |
| Thermal stability at 37°C | > 30 days |
For small-scale trials, a simple stirrer at approximately 300-500 RPM is recommended.
The emulsion appears milky white, with a slight pale blue tint on the walls when shaken.