Instructions for using the LanJieKe Mouse IgG Total ELISA Kit (BSEM-059-48T/96T) to measure IgG concentrations in mouse samples.
This manual provides an overview of the Mouse IgG Total ELISA Kit, including its components, detection principle, and recommended storage conditions. It explains how to collect, process, and store mouse serum, plasma, or cell culture supernatant samples before analysis.
Detailed step‑by‑step procedures cover reagent preparation, plate incubation, washing, color development, and result interpretation. Troubleshooting tips and common questions are included to help achieve reliable quantitative results. This manual is written in Chinese.
| Detection range | 0 – 100000 pg/mL |
|---|---|
| Sensitivity | < 780 pg/mL |
| Storage temperature (unopened) | 2‑8 °C |
| Shelf life (unopened) | 6 months |
| Use after opening | Within 1 month |
The kit uses a double‑antibody sandwich ELISA where mouse IgG total is captured by a coated monoclonal antibody, followed by a labeled detection antibody and color development.
Keep the unopened kit at 2‑8 °C for up to six months; after opening, store at 2‑8 °C and use within one month.
Mouse serum, plasma, or cell culture supernatant can be used for IgG total quantification.