The user manual for Beijing Juhui Mei's M5 HiPer Supermicroscale Protein Stain explains storage, usage, and safety for SDS-PAGE and native gels.
M5 HiPer Supermicroscale Protein Stain is a non‑toxic, non‑heating protein gel stain based on a novel ion‑pair dye technology. It provides high sensitivity (minimum 0.4 ng) and low background without the need for gel fixation or de‑staining.
The manual details storage conditions (room temperature, protected from light for 12 months), recommended volumes (100 ml MF768‑01 or 500 ml MF768‑05), and step‑by‑step staining procedures for SDS‑PAGE and native gels, including cautions and reuse guidelines. This manual is written in Chinese.
| Sensitivity | ≥0.4 ng |
|---|---|
| Linear Range | 0.4–1500 ng |
| Storage Condition | Room temperature, protected from light, 12 months |
| Available Volumes | 100 ml (MF768‑01) and 500 ml (MF768‑05) |
| Solvent-Free | No methanol, acetic acid, trichloroacetic acid |
Typical staining completes in 3–20 minutes; for very low‑amount proteins, staining may be extended to 30 minutes‑2 hours.
Yes, the solution can be reused up to three times; staining speed may slow slightly but sensitivity remains unchanged.
No, the stain does not require gel fixation or de‑staining; the background stays low and bands are visible directly.
It works with SDS‑PAGE gels (both homemade and pre‑cast) and native (non‑denaturing) gels, as well as residual proteins on membranes after Western transfer.