The JinMing Biotechnology 1×PonceauS staining solution manual provides usage instructions, advantages, and safety notes for protein detection on PVDF, NC…
The 1×PonceauS staining solution (丽春红S染色液) is a water‑soluble anionic azo dye used for reversible protein detection on PVDF, nitrocellulose (NC) and cellulose acetate membranes. It produces red bands on a pink background and is not suitable for nylon membranes.
The manual describes the composition, storage conditions (room temperature, protected from light), and step‑by‑step staining procedure, including washing with distilled water or PBS and safety precautions such as wearing a lab coat and disposable gloves. The solution is stable for 12 months and can detect proteins of 250 ng or more. This manual is written in Chinese.
| Molecular formula | C22H12N4Na4O13S4 |
|---|---|
| Molecular weight | 760.6 |
| Storage condition | Room temperature, protected from light |
| Detection limit | ≥250 ng protein |
| Shelf life | 12 months |
It can be used on PVDF, nitrocellulose (NC) and cellulose acetate membranes.
Yes, the stain can be removed by washing with distilled water, PBS, or other suitable solutions.
No, it is not suitable for protein detection on nylon membranes.
Wear a lab coat and disposable gloves during operation.