The Jianshi Bio TRIcomReagent manual provides step‑by‑step instructions for extracting total RNA from various biological samples.
This manual describes the use of TRIcomReagent for total RNA extraction from human, animal, plant, yeast, bacterial and viral samples. It includes safety warnings, storage conditions, and detailed protocols for tissue, cell and blood processing.
Key steps cover sample homogenization, phase separation with chloroform, RNA precipitation with isopropanol, ethanol washes and resuspension. Safety precautions such as wearing lab coat, goggles and gloves, and handling the corrosive, flammable reagent are emphasized throughout the procedure. This manual is written in Chinese.
| Catalog Number | TR201-50 (50 ml) / TR201-100 (100 ml) |
|---|---|
| Storage Temperature | 2–8 °C |
| RNA Precipitate Storage | 75 % ethanol, 2–8 °C up to 1 week or –20 °C up to 1 year |
Store the reagent at 2–8 °C, protected from light, and use it within 9–12 months.
Wear a lab coat, safety goggles and gloves; avoid skin contact and ingestion, and rinse immediately with detergent and plenty of water if contact occurs.
Low yield can result from incomplete sample lysis or homogenization, insufficient reagent volume, or incomplete dissolution of the RNA pellet.