This manual provides usage instructions for Immunoway Antigen Retrieval Buffer (20X) EDTA pH9.0, a reagent designed to improve immunostaining results on…
The document describes the Immunoway Antigen Retrieval Buffer (20X) EDTA pH9.0, intended for research use only. It is used to remove cross-links caused by aldehyde fixation in cell or tissue samples, thereby exposing antigen epitopes and improving immunostaining signals on paraffin sections and potentially frozen sections.
The manual details the preparation of a 1X working solution from the 20X concentrate using water at a 19:1 ratio. It provides specific protocols for antigen retrieval using both microwave heating (95-100°C for approximately 20 minutes) and high-pressure autoclaving methods, including steps for washing and cooling. This manual is written in Chinese.
Store at 2°C to 8°C in a sealed container.
The product has an expiration period of two years from the date of manufacture.
Mix 20X concentrate with water at a volume ratio of 19:1 to prepare the 1X solution.