IBA One-STrEP Starter Kit isolates protein complexes in one step under physiological conditions with low background.
The One-STrEP Starter Kit is designed to identify protein complexes by isolating transiently and weakly interacting proteins in a single purification step. It uses Strep-Tactin columns and includes plasmids, buffers, and controls for verification. The kit is suitable for identifying multiple binding partners or cofactors simultaneously.
Components include plasmids for bait cloning, Strep-Tactin columns, buffers, and detection antibodies. A positive control system with PR65 and PP2Ac subunits ensures successful isolation of intact protein complexes.
| Purification Steps | One STEP |
|---|---|
| Background | Low background |
| Binding Partners | Several binding partners/co-factors |
Only one step is required to isolate intact protein complexes.
The kit includes plasmids for bait cloning, Strep-Tactin columns, buffers, and control components for verification.
A positive control system with PR65 and PP2Ac subunits confirms successful isolation of an intact protein complex.