This manual describes the Huankai RV Salmonella Enrichment Broth (Model 023022 P1), a selective liquid medium for microbiological testing of Salmonella species.
The document provides instructions and specifications for the Huankai RV Salmonella Enrichment Broth, product number 023022 P1. It is designed for the selective enrichment culture of Salmonella bacteria in food safety monitoring.
The manual details the composition per liter, including soy peptone, magnesium chloride, sodium chloride, phosphates, and孔雀绿 (malachite green) to inhibit non-Salmonella growth. It outlines preparation steps involving dissolving 27.1g of powder in 1L of water, sterilization at 115°C for 15 minutes, and storage conditions. This manual is written in Chinese.
| Soy Peptone Content | 4.5 g/L |
|---|---|
| Magnesium Chloride Content | 13.58 g/L |
| Sodium Chloride Content | 8.0 g/L |
| Final pH | 5.2±0.2 |
| Preparation Amount | 27.1g per liter |
| Sterilization Condition | 115°C for 15 min |
| Shelf Life (Unopened) | 3 years |
It is used for the selective enrichment culture of Salmonella bacteria.
Dissolve 27.1g in 1L of distilled or deionized water by heating and boiling until completely dissolved.
The prepared medium should be autoclaved at 115°C for 15 minutes.