Instructions for the Fuller Labs RTG-96K kit, an indirect enzyme immunoassay for detecting IgG antibodies against Rickettsia typhi in human serum or plasma.
The Fuller Labs RTG-96K is an indirect enzyme immunoassay intended for in-vitro diagnostic use to detect and semi-quantitate IgG class human antibody to Rickettsia typhi. The kit utilizes species-specific protein (rOmp B) and lipopolysaccharide antigen purified from Rickettsia typhi coated on EIA microwells.
| Test Volume | 96 tests |
|---|---|
| Storage Temperature | 2-8˚C |
| Incubation Time (Step 1) | 60 minutes |
| Incubation Time (Step 2) | 30 minutes |
| Reaction Time | 10 minutes |
| Reading Wavelength | 450nm |
The kit is intended for the detection and semi-quantitation of IgG class human antibody to Rickettsia typhi, to be used as an aid in the diagnosis of human infection by this pathogen.
The absorbance value must be less than 0.2 units.
Indices above 1.2 are considered positive, while indices below 0.8 are negative.