Standard operating procedure for the FEI Quanta 200 Scanning Electron Microscope, covering specimen preparation, startup, operation, and shutdown.
This document serves as a guide for certified users of the FEI Quanta 200 Scanning Electron Microscope. It details the standard operating procedures, including specimen preparation and handling, computer interface usage, and detector management. The guide explains the three operating vacuum modes: High Vacuum, Low Vacuum, and ESEM, providing specific instructions for each based on sample type.
| Operating Vacuum Modes | High Vacuum, Low Vacuum, ESEM |
|---|---|
| Low Vacuum Pressure Range | 0.1 to 30 Torr |
| Accelerating Voltage Range | 0-30 kV |
| Working Distance | approximately 10 mm |
| Recommended Voltage for Polymers | 2-6 kV |
| Recommended Voltage for Metals | 10-20 kV |
| Working Pressure Limit | 5x10-5 torr or below |
| Image Resolution Size | 1024x884 |
ESEM mode is primarily used for specimens that are considered 'wet' and contain volatile components such as water or oil.
A voltage between 2-6 kV works well for polymer and glass samples.
Users must wear latex gloves when handling detectors or loading specimens.