The Dakewei DC-specific Cytokine Kit manual describes the kit components, preparation ratios, and step‑by‑step cell culture procedures for human dendritic…
The DC‑specific Cytokine Kit (DC‑CYT) is designed as an induction activator for human dendritic cell (DC) culture. It contains two components, Component I and Component II, which are added to serum‑free or serum‑containing media at a 1:100 ratio.
The manual provides detailed culture steps, including cell isolation, media preparation, addition of the kit at specific volumes (e.g., 50 µL per 5 mL medium), incubation conditions, and subsequent supplementation on days 3, 5, and 6. Storage instructions require keeping the kit at –20 °C to –80 °C and avoiding repeated freeze‑thaw cycles. The product is intended for research use only. This manual is written in Chinese.
| Recommended storage temperature | -20 °C to -80 °C |
|---|---|
| Shelf life | 12 months |
Add each component at a 1:100 ratio, e.g., 50 µL of Component I or II per 5 mL of medium.
Store the kit at –20 °C to –80 °C and avoid repeated freeze‑thaw cycles.
No, the kit is for research use only.