This manual provides comprehensive guidance on zebrafish biology, breeding, disease management, genetics, and transgenic techniques for researchers.
The training manual was prepared by the China Zebrafish Resource Center (CZRC) and the National Aquatic Biological Resource Center (NABRC) for the 15th nationwide zebrafish experimental techniques conference. It offers systematic instruction on zebrafish basic information, development, research status, genetics, breeding systems, disease control, and advanced genetic manipulation methods.
The document is organized into five main chapters: (1) basic information on the model animal zebrafish, (2) construction and operation of zebrafish housing and laboratory breeding, (3) common fish diseases and health maintenance, (4) gene mutation techniques and identification methods, and (5) various transgenic technologies and their identification, followed by detailed SOP appendices. This manual is written in Chinese.
| Optimal growth temperature | 20‑30 °C |
|---|---|
| Standard embryo culture temperature | 28.5 °C |
| Adult length | 3‑5 cm |
A healthy adult pair can produce more than 200 eggs in a single mating.