Bruker RapiFlex MALDI-TOF User Manual

About this manual

The Bruker RapiFlex MALDI‑TOF manual provides step‑by‑step instructions for qualified users at the Integrated Molecular Structure Education and Research Center (IMSERC). It outlines safety policies, data‑folder setup, software operation (FlexControl and FlexAnalysis), and detailed sample‑preparation guidelines.

The document also includes pre‑run and quick‑start checklists, data‑acquisition workflow, mass‑list generation, troubleshooting tips for NUCore reservations, and contact information for IMSERC staff. Appendices cover advanced operations and revision history.

What's inside

• Introduction
• Safety
• Data Management
• Software
• Sample Preparation
• Pre‑Run Checklist
• Quick Start Acquisition Checklist
• Data Acquisition – Overview
• Data Analysis
• Troubleshooting

Frequently asked questions

How do I turn on the instrument screen?

You must begin a reservation in NUCore; the screen will activate once the reservation starts.

What personal protective equipment is required in the MALDI‑TOF room?

Protective eyewear is required, and gloves should be removed when using the computer.

Where are the acquired data files stored?

Data are copied to imsercdata.northwestern.edu under the MS/MALDI‑Rapiflex directory.

How can I generate a mass list from my spectrum?

Click the Mass List icon for automatic generation, or use the Edit Mass List icon to label peaks manually.