This user manual explains how to use the Bowei Biotechnology food proof® GMO 1 lyophilized kit for qualitative detection of P‑35S, T‑NOS and P‑FMV sequences…
The food proof® GMO 1号试剂盒（冻干粉） is a 96‑reaction lyophilized PCR kit designed to detect the P‑35S, T‑NOS and P‑FMV GMO sequences in food, feed, raw material and seed samples. The kit includes pre‑filled reaction wells, positive and negative control tubes, and all reagents needed for DNA amplification and detection.
The manual details storage conditions (2 °C‑8 °C), preparation of the PCR mix, instrument programming for compatible real‑time PCR devices, and step‑by‑step procedures for adding samples, controls, and running the assay. It also provides troubleshooting tips for common issues such as lack of fluorescence signal or contamination. This manual is written in Chinese.
| Number of reactions | 96 (up to 94 samples + controls) |
|---|---|
| Final reaction volume | 25 µL |
| Storage temperature | 2 °C to 8 °C |
| Detection channels | FAM, VIC/HEX, ROX, Cy5 |
| Compatible PCR instruments | LightCycler® 480, LightCycler® 96, Mx3005P®, ABI 7500 FAST, AriaMx®, PikoReal® 24 |
The kit must be stored between 2 °C and 8 °C.
The kit is designed for 96 reactions, allowing up to 94 samples plus positive and negative controls.
The kit works with real‑time PCR instruments that can detect FAM, VIC/HEX, ROX and Cy5 channels.