This manual describes the BioTeke FastSuperRTKit, a rapid reverse transcription system containing gDNase to remove genomic DNA contamination for PCR and…
The BioTeke FastSuperRTKit is an efficient and fast reverse transcription system designed to synthesize cDNA first strands while removing genomic DNA contamination. It contains gDNase and M-MLV Reverse Transcriptase, allowing for high-efficiency synthesis (over 90%) even with complex RNA templates containing high GC content or secondary structures. This manual is written in Chinese.
| Storage Temperature | -20°C |
|---|---|
| Reverse Transcription Efficiency | over 90% |
| Template RNA Range | 0.2-2 μg |
| Reverse Transcription Temperature 1 | 50°C |
| Reverse Transcription Temperature 2 | 72°C |
| gDNase Digestion Time | 3 min (efficient) |
| Storage Duration at -20°C | 1 year |
The synthesis efficiency reaches over 90%.
Yes, it can read through RNA templates with high GC content and complex secondary structures.
The template amount (Total RNA) should be between 0.2 and 2 μg.
The efficient removal of genomic DNA takes 3 minutes.