BioTek Synergy 2 Setup Guide for Predictor hERG FP Assay

About this manual

This document provides a setup guide for the BioTek Instruments Synergy™ 2 Multi-Mode Microplate Reader specifically for use with the Predictor™ hERG FP Assay. It details the recommended optics configuration, including excitation and emission filters, and provides step-by-step instructions for configuring the Gen5 software protocol.

The guide also outlines the experimental procedure for preparing the assay plate, including compound dilution, control preparation (negative, positive, free tracer, and blank), and the incubation steps required before reading the plate.

What's inside

• Recommended Optics
• Instrument Setup
• Gen5 Software Configuration
• Plate Layout
• Compound Preparation
• Control Preparation
• Assay Procedure

Specifications

Frequently asked questions

How should the read speed be set for best results?

The read speed should be set to 150 measurements; using fewer measurements may decrease read time but could adversely affect assay performance.

What specific assay is this setup guide intended for?

This guide demonstrates the setup of the BioTek Instruments Synergy™ 2 Multi-Mode Microplate Reader for the Predictor™ hERG FP Assay.

What volume of compound should be added per well?

You must add 5 µl of compound per well to the untreated Corning 3677 black polystyrene 384-well plate.

How long should the plate be incubated before reading?

After shaking the plate on an orbital plate shaker for 30 seconds, you must incubate it for 2 hours before reading.