The BIOO 1030-02 ELISA test kit manual provides product description, protocol, storage, sensitivity, and troubleshooting for aflatoxin analysis.
This manual describes the MaxSignal® Total Aflatoxin ELISA Test Kit, a competitive enzyme immunoassay for quantitative analysis of aflatoxins in food and feed samples. It includes product description, kit contents, storage conditions, detection limits, cross‑reactivity data, required equipment, sample preparation steps, the full ELISA protocol, and troubleshooting guidance.
The kit is designed for laboratory use, supports up to 96 determinations (42 samples in duplicate), and must be stored at 2‑8 °C with a 12‑month shelf life. Detailed instructions cover reagent preparation, plate handling, incubation times, washing steps, and plate reading at 450 nm.
| Detection limit (dried meat/fish, cereal, feed, seed) | 0.5 ng/g or ppb |
|---|---|
| Detection limit (meat/liver/kidney) | 0.5 ng/g or ppb |
| Detection limit (milk) | 0.5 ng/g or ppb |
| Cross‑reactivity – Aflatoxin B1 | 100 % |
| Cross‑reactivity – Aflatoxin B2 | 76 % |
| Cross‑reactivity – Aflatoxin M1 | 79 % |
| Shelf life | 12 months (when stored properly) |
| Recommended storage temperature | 2‑8 °C |
| Assay capacity | 96 determinations (42 samples in duplicate) |
The detection limit is 0.5 ng/g or ppb for those sample types.
Store the kit at 2‑8 °C; it remains stable for 12 months when stored properly.
Incubate plates at room temperature, 20‑25 °C (68‑77 °F).