The BioChain Eva QPCR SuperMix Kit manual provides instructions, protocols, and specifications for using the K5052200 and K5052400 kits.
The BioChain Eva QPCR SuperMix Kit manual introduces the ready‑to‑use 2× master mix that contains Eva Dye, hot‑start Taq polymerase, ROX reference dye, and a human β‑actin primer pair for quantitative real‑time PCR.
It provides detailed instructions on storage, reagent preparation, ROX dye usage, PCR reaction setup, cycling programs, dissociation steps, and troubleshooting, along with specifications and related product information.
| Concentration | 2x-concentrated master mix |
|---|---|
| Fluorescent dye | Eva Dye |
| Reference dye | ROX reference dye included |
| Control primer | human β‑actin primer pair |
| Assay capacity (K5052200) | 200 assays |
| Assay capacity (K5052400) | 400 assays |
| Storage stability | stable for 6 months at -20 °C |
The K5052200 kit provides reagents for 200 assays, while the K5052400 kit provides reagents for 400 assays.
Store all components at –20 °C and avoid repeated freeze‑thaw cycles; once thawed, the mix is stable for at least 3 months at 4 °C.
ROX dye can be added to compensate for fluorescence variations; dilute 1:10 for compatible instruments and add 0.5 µl per 25 µl reaction, or use undiluted ROX for other instruments.
The kit can detect as few as 20 copies of target DNA within the linear range.