The Beyotime CellTiter‑Lumi™ Plus II kit provides a high‑sensitivity, wide‑range luminescent assay for measuring cellular ATP and viability.
The CellTiter‑Lumi™ Plus II luminescent cell viability assay kit (CTL Plus II) uses a chemical‑luminescence method to quantify intracellular ATP, offering ultra‑high sensitivity and a broad linear range for cell counting. It is supplied as a lyophilized powder that is reconstituted with the provided buffer, allowing stable storage at –20 °C.
The kit includes reagents for 96‑well or 384‑well plates, detailed usage steps, storage guidelines, and performance data such as linear range, detection limits, and signal stability. It is intended for research use only and must be handled with standard laboratory safety precautions. This manual is written in Chinese.
| Linear range (HeLa cells) | 10‑100,000 cells per well |
|---|---|
| Detection sensitivity | Detects as few as 25 cells per well |
| Storage temperature | -20 °C (light‑protected) for ≥2 years |
| Assay volume (96‑well) | 200 µL total (100 µL cells + 100 µL reagent) |
| Incubation time | ≈10 minutes |
| Plate type | White or black 96‑well or 384‑well plates |
The reagents remain stable for at least two years when stored at –20 °C in the dark.
Use 100 µL of cell culture and 100 µL of reagent per well, for a total of 200 µL.
A signal twice the blank can be obtained with as few as 25 cells per well.
White or black 96‑well or 384‑well plates should be used; transparent plates may cause cross‑talk.