Product leaflet for Ambion RNA Millennium Markers AM7150, a set of 10 RNA transcripts used as size standards for Northern blots.
Ambion RNA Millennium Markers (Catalog # AM7150) are a set of 10 transcripts with lengths ranging from 0.5 to 9 kb, each containing a 20 base poly(A) tail. They are designed for use as RNA size standards in Northern blots and can be visualized by ethidium bromide staining, UV fluorescence, or autoradiography. The product contains 25 lanes at a concentration of 1.0μg/μL in a volume of 50μL.
| Transcript Lengths | 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6 and 9 kb |
|---|---|
| Poly(A) Tail | 20 base |
| Concentration | 1.0μg/μL |
| Volume | 50μL |
| Storage Temperature | below –70 °C |
| Storage Buffer | 0.1 mM EDTA |
| Lanes | 25 |
Two microgram (2 μg) of RNA Millennium Markers should be enough to visualize within the gel.
Yes, they can be visualized by autoradiography using the Ambion Millennium Marker Probe (P/N AM7785).