User manual for Alpha Diagnostic Intl's Ampicillin ELISA Kit DE-100140, for quantitative detection in pork liver, chicken, duck, shrimp, fish, honey, and milk.
This document provides instructions for using the Ampicillin ELISA Kit DE-100140 to detect ampicillin in various food samples. It includes kit components, testing procedures, calculation methods, and technical specifications. The kit uses a competitive enzyme immunoassay method and requires a microplate reader and standard solutions for accurate results.
Precautions are outlined to ensure proper handling and storage of reagents, including avoiding light exposure, maintaining temperature, and using only disposable tips to prevent contamination.
| Sensitivity | 0.1 ppb |
|---|---|
| Detection Limit | 2 ppb (for chicken, duck, pork, liver, fish, shrimp) / 4 ppb (for honey, milk) |
| Recovery Rate | 75%±10% (for chicken, duck, pork, liver, fish, shrimp) / 70%±10% (for honey, milk) |
| Cross-Reaction Rate | Ampenicillin 100%, Benzyl penicillin 0.8%, Cloxacillin 0.2%, Dicloxacillin 0.1%, Amoxicillin 0.1%, Ceftiofu 0.1% |
The detection limit for honey and milk is 4 ppb due to potential interference.
The concentration is calculated by comparing the sample's OD value to a standard curve, using either rough judgment or quantitative determination methods.
Cross-reactivity rates include: Ampenicillin 100%, Benzyl penicillin 0.8%, Cloxacillin 0.2%, Dicloxacillin 0.1%, Amoxicillin 0.1%, Ceftiofu 0.1%.