Technical data sheet for BioWest L0430 Minimum Essential Media with Earle’s salts, non‑essential amino acids and no L‑glutamine, covering composition…
About this manual
The BioWest L0430 Minimum Essential Media (MEM) is a cell‑culture medium formulated with Earle’s balanced salts, non‑essential amino acids and without L‑glutamine. It is intended for in‑vitro laboratory use only and supports the growth of a variety of cell lines, including L929 fibroblasts.
The medium should be stored at +2 °C to +8 °C, protected from light, and has a shelf life of 24 months. It is supplied as a red‑orange solution with a theoretical pH of 7.3 ± 0.3 and an osmolality of 290 mOsm/kg ± 10 %. Sterility tests confirm the absence of bacteria, fungi and yeasts, and endotoxin levels are below 1 EU/ml.
What's inside
- Description
- Composition
- Recommended Use
- Storage Conditions
- Shelf Life
- Sterility Tests
- Endotoxin Limit
- Cell Growth Test
- Signs of Deterioration
- Component List
Specifications
| Theoretical pH | 7.3 ± 0.3 |
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| Osmolality | 290 mOsm/kg ± 10 % |
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| Colour | Red, orange solution |
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| Storage temperature | +2 °C to +8 °C, protected from light |
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| Shelf life | 24 months |
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| Endotoxin limit | < 1 EU/ml |
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| Sterility test scope | Bacteria (aerobic & anaerobic), fungi and yeasts |
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| Cell growth test | Supports L929 cell growth |
Frequently asked questions
What are the recommended storage conditions for BioWest L0430 MEM?
Store the medium at +2 °C to +8 °C and keep it protected from light.
How long can BioWest L0430 MEM be kept before it expires?
The medium has a shelf life of 24 months when stored under the recommended conditions.
What is the theoretical pH of this medium?
The theoretical pH is 7.3 ± 0.3.
Can this medium be used for therapeutic or veterinary applications?
No, it is intended for in‑vitro laboratory use only and must not be used in therapy, human or veterinary applications.