OriCiro ® Cell-Free Switching System

OriCiro® Cell-Free Switching System allows you to switch the plasmid DNA amplification from using living cells to cell-free system. SS OriC cassette is inserted into your plasmid DNA via homology arms designed to target a conserved sequence flanking replication origin of popular vector plasmids*. The resultant oriC-containing plasmid is then amplified in our unique cell-free reaction as a supercoiled form without the need of cumbersome cloning relying on E. coli.

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