This document describes the immunostaining reagent I20012 series, its intended use in immunohistochemistry or in situ hybridization, and the principle of DAB chromogenic detection. It lists the reagent components, packaging specifications, and storage requirements.
Detailed step‑by‑step procedures cover antigen retrieval, blocking, primary antibody incubation, secondary antibody application, DAB development, counter‑staining, and final slide preparation. Safety precautions, waste disposal, and interpretation of staining results are also included. This manual is written in Chinese.
| Package size (I20012A) | 50 tests per box |
|---|---|
| Reagent A volume | 5 mL |
| Storage temperature | 2‑8 °C |
| Shelf life | 18 months (use within 3 months after opening) |
Mix 0.5 mL of DAB substrate (Reagent C) per milliliter of DAB buffer (Reagent D) at a ratio of about one drop per milliliter.
Store the reagent at 2‑8 °C and use it within three months after opening; the overall shelf life is 18 months.