This document is the operation manual for JEOL 2500SE (S)TEM, which includes the description of various parts of the microscope, overall schematic, side entry specimen holder, control panels, and graphic user interface. It also provides procedures for sample loading, column alignment, condenser alignment, eucentric height adjustment, stigmation adjustment, tilt balance, axis align, image focus, finding the zone axis, objective astigmatism correction, image capture, camera setup, image search and capture, HRTEM image capture, STEM mode, EELS, and sample unloading.
The document serves as an operation guide for the JEOL 2500SE (S)TEM. It details the fundamental principles of electron microscopy and describes the physical parts of the microscope, including the side entry specimen holder and control panels.
The core of the manual consists of step-by-step procedures for operating the instrument. These instructions cover essential tasks such as sample loading, column alignment, condenser alignment, Eucentric height adjustment, stigmation correction, tilt balance, axis alignment, image focus, finding the zone axis, objective astigmatism correction, and capturing images in both TEM and STEM modes.
Switch to PUMP on the goniometer assembly to activate the vacuum pumps.
An aperture size of 2 is typically selected on the drop-down menu.
Adjust the Fourier transform image until the rings become circular using the multifunction XY knobs.
Go to Custom and select Save as HR-TIFF.