This document describes the implementation of the Edman degradation chemistry on Hewlett-Packard's column-based protein sequencer and its applicability for N-terminal amino acid sequence analysis of most protein and peptide samples in the nanomole to low picomole range.
This document details the Routine 3.1/3.5 sequencer methods used with Hewlett-Packard's column-based protein sequencer, specifically model G1005A. These methods implement Edman degradation chemistry for N-terminal amino acid sequence analysis of protein and peptide samples in the nanomole to low picomole range.
| Sample range | nanomole to low picomole range |
|---|---|
| Routine 3.1 column method time | 43.9 min (2631.6 sec) |
| Routine Cycle 1 time | 58.7 min (3520.1 sec) |
| Routine 3.5 column method time | 42.0 min (2518.0 sec) |
| Waste volume per cycle | less than 1mL/cycle |
The G2046A kit is purchased from Hewlett-Packard for the Routine 3.1 method.
The G2021A kit is purchased from Hewlett-Packard for the Routine 3.5 method.
It is used to wash the sequencer column prior to sample loading, which is especially important for low-level samples.