This document introduces the Agilent 1200 Infi nity Series High Dynamic Range Diode Array Detection Solution, which provides a 30× wider linear dynamic UV range. By combining the signals from two diode array detectors with different path length Max-Light fl ow cells, the high dynamic range DAD solution detects and quantifies main and trace compounds in a single run without exceeding the linear UV range. Using a conventional DAD, typically two injections with different injection volumes are needed to determine the low dose and high dose as well as the trace compounds. At the high injection volumes that are required to determine the low dose and trace compounds, the high dose compounds exceed the linear range of a conventional detector. This Technical Overview demonstrates that one single injection is adequate to reliably quantify low dose, high dose, and trace compounds of a fixed dose combination drug using the high dynamic range detection solution from Agilent. The results are compared to those from a conventional DAD. In addition, the precision of areas and limit of detection for both detection solutions are evaluated and compared.
The document presents a technical evaluation of the Agilent 1200 Infinity Series High Dynamic Range (HDR) Diode Array Detection Solution. This solution combines signals from two diode array detectors with different path lengths to achieve a 30x wider linear dynamic UV-range, allowing for the detection and quantification of main and trace compounds in a single run.
The linear UV-range is typically as wide as 0.6 x 10^5 to 6.7 AU/cm.
The HDR solution allows for reliable quantification of low dose, high dose, and trace compounds in one single injection, whereas a conventional DAD typically requires two injections with different volumes.