StrataClone™ PCR Cloning Systems is a high-performance PCR cloning system using Stratagene's DNA Topoisomerase I Technology. The system allows cloning of both long and short amplicons and provides kits for both UA and blunt-end cloning. With just 5 minutes from PCR to transformation, high efficiency yields >95% clones with insert. The multiple cloning site offers more restriction enzyme sites.
Stratagene's StrataClone PCR Cloning Systems utilize DNA Topoisomerase I technology to facilitate the cloning and characterization of genes. These systems allow users to clone both long and short amplicons with high efficiency, yielding over 95% clones with the insert. The process is designed to be fast and reliable, requiring only a simple three-step protocol that takes just five minutes from PCR to transformation, eliminating the need for PCR clean-up steps.
| Cloning Efficiency | >95% |
|---|---|
| Time from PCR to Transformation | 5 minutes |
| Cloning Steps | Three-step protocol |
| PCR Clean-up Requirement | None |
| Cloning Types Supported | UA and blunt-end |
| Amplicon Length Support | Long and short |
The process takes just 5 minutes from PCR to transformation.
No, PCR clean-up is not required as the system uses a simple three-step process.
The system provides high efficiency yields of greater than 95% clones with the insert.
Yes, the kits are available for both blunt-end and UA cloning.