The document introduces Agilent's novel and powerful method of location analysis of DNA binding proteins. This method combines chromatin immunoprecipitation (ChIP) with Agilent DNA microarrays to construct comprehensive DNA-protein binding profiles. The product features comprehensive and genome-wide coverage, superior microarray performance with optimized 60-mer oligonucleotide probes and a convenient two-color labeling system, and reliable binding data generated by powerful algorithms employing neighborhood probe voting with multiple probes to generate reliable data with greater true-binding events and fewer false positives.
The Agilent G4495A Human ENCODE ChIP-on-Chip Set utilizes proprietary SurePrint technology to enable the location analysis of DNA-binding proteins. It combines chromatin immunoprecipitation (ChIP) with a high-density oligonucleotide microarray to construct comprehensive profiles of human gene regulation across the ENCODE regions on a genome-wide scale.
The set features 153,000 probes covering chromosomes 1-22 and includes optimized 60-mer oligonucleotide probes. It is designed for use with Agilent ChIP Analytics software to provide reliable binding data through neighborhood probe voting algorithms.
| Probe Coverage | Chromosomes 1-22 (~153,000 probes) |
|---|---|
| Probe Length | 60 bases |
| Starting Sample Input | 0.5x 10^8 cells |
| Type of Labeling | Random priming using Klenow with Cyanine-3 and -5 nucleotides |
| DNA Required for Hybridization | 5 ug per channel |
It is used to construct comprehensive DNA-protein binding profiles by combining chromatin immunoprecipitation with Agilent DNA microarrays.
The set covers chromosomes 1-22 with approximately 153,000 probes.
The procedure requires 0.5 x 10 to the power of 7 cells as the starting sample input.