This document introduces Agilent's method for location analysis of DNA binding proteins. This method uses chromatin immunoprecipitation (ChIP) and Agilent DNA microarrays to construct comprehensive DNA-protein binding profiles.
The Agilent G4490A Mouse Promoter Arrays ChIP-on-Chip Set is a novel method combining chromatin immunoprecipitation (ChIP) with DNA microarrays to analyze protein-DNA binding profiles. This technology allows for the construction of comprehensive DNA-protein binding profiles on a genome-wide scale, providing broad insights into mouse gene regulation mechanisms.
| Probes coverage | ~17,000 of the best defined mouse transcripts |
|---|---|
| Probe length | 60 bases |
| Minimum order | 5 sets (10 slides) |
| Starting sample input | 0.5 x 10-1 x 10° cells |
The probes cover approximately 17,000 of the best defined mouse transcripts and span from -5.5 KB upstream to +2.5 KB downstream from transcriptional start sites.
The arrays are printed using Agilent's SurePrint technology, which features a flexible, industrial-scale inkjet printing process.
The set is compatible with ChIP Analytics software, which combines annotated data processing with statistical modeling functions.