Poroshell 300SB is a high-resolution, high-sensitivity, and high-speed protein laboratory reagent, suitable for situations with limited sample size, a large number of samples, and a need for rapid separation of contaminants. It provides ultra-sharp peaks in a short run time and requires less sample. Increased temperature allows for shorter run times, increased resolution by sharpened peaks, and reduced mobile-phase viscosity for maximal throughput.
This document discusses the application of Poroshell 300SB-C18 columns in protein laboratories where sensitivity, speed, and resolution are critical. It highlights how these columns deliver ultrasharp peaks with ultrashort run times and require less sample volume compared to conventional 4.6-mm id columns.
The text explains that increased temperature enables shorter run times, increased resolution through sharpened peaks, and reduced mobile-phase viscosity for maximal throughput. Specific chromatographic conditions and results for analyzing Neurotensin, RNase A, Lysozyme, and Myoglobin at temperatures up to 70°C are presented.
| Column Dimensions | 2.1 x 75 mm |
|---|---|
| Particle Size | 5 pm |
| Part Number | 660750-902 |
| Flow Rate | 2 mL/min |
| Column Pressure | 195 bar |
| Mobile Phase A | 95% H2O, 5% ACN with 0.1% TFA |
| Mobile Phase B | 5% H2O, 95% ACN with 0.07% TFA |
They deliver ultrasharp peaks with ultrashort run times and require less sample (20% that of a 4.6-mm id column).
Increased temperature enables shorter run times, increased resolution by sharpened peaks, and reduced mobile-phase viscosity for maximal throughput.