Agilent HybriZAP-2.1 Two-Hybrid Undigested Vector Kit Instruction Manual

This manual introduces the use of HybriZAP-2.1 Two-Hybrid Undigested Vector Kit.


Agilent HybriZAP-2.1 Two-Hybrid Undigested Vector Kit Instruction Manual - cover page
Brand
HybriZAP-2.1
Document type
User Manual
Language
English
Pages
72
File format
PDF
File size
525 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
C875BECC0CDE2000270794AD04580E62

About this manual

The document serves as an instruction manual for the HybriZAP-2.1 Two-Hybrid Undigested Vector Kit (Catalog #211341). It details the materials provided, including vectors, control plasmids, bacterial and yeast host strains, and helper phage. The kit is designed for in vitro use to facilitate the identification of protein-protein interactions.

What's inside

  • Materials Provided
  • Storage Conditions
  • Additional Materials Required
  • Bacterial Host Strains
  • Yeast Host Strain
  • Helper Phage
  • Control Plasmids
  • Activation Domain Vector Construction
  • DNA-Binding Domain Vector Construction
  • Troubleshooting

Specifications

Vector Concentration (HybriZAP-2.1)1 ug/l
Volume of HybriZAP-2.1 Vector20 ug
Bacterial Strain NameXL1-Blue MRF'
Yeast Host Strain NameYRG-2

Frequently asked questions

What is the catalog number for this kit?

The catalog number for the HybriZAP-2.1 Two-Hybrid Undigested Vector Kit is 211341.

What are the storage conditions for the vectors?

Vectors should be stored at -20°C. For short-term storage, they can be kept at 4°C for one month.

What is the recommended temperature for storing bacterial glycerol stocks?

Bacterial glycerol stocks should be stored at -80°C.

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