InterPlay Mammalian TAP System INSTRUCTION MANUAL is a product manual released by Agilent, which details the use of InterPlay N-terminal Mammalian TAP System, InterPlay C-terminal Mammalian TAP System, InterPlay N-terminal Mammalian TAP Vectors, InterPlay C-terminal Mammalian TAP Vectors, InterPlay TAP Purification Kit, and InterPlay TAP Purification Buffer Kit.
The document serves as an instruction manual for the InterPlay Mammalian TAP System (Catalog #240103/240104), a kit designed for in vitro research use. It details the materials provided, including specific expression vectors (pNTAP/pCTAP) and purification reagents like Streptavidin and Calmodulin resins.
The guide outlines a complete workflow starting with vector preparation and ligation of inserts, followed by transformation and verification steps. It further describes mammalian cell transfection procedures for both experimental and control vectors. The latter half of the manual focuses on the TAP protocol, detailing how to prepare protein extracts using specific resins and purify protein complexes via Streptavidin and Calmodulin affinity chromatography.
| Resin Volume (Streptavidin) | 1.25 ml settled / 2.5 ml total slurry |
|---|---|
| Purification Capacity | Sufficient for 5 purifications (1 x 10^8 cells/purification) |
All vectors should be stored at -20°C upon receipt.
No, the product is strictly for research use only and not for clinical, therapeutic, or diagnostic use.